菌根真菌提高植物抗旱性机制的研究回顾与展望.pdf

Review 特约综述 22 April 2021 40 4 851 872 Mycosystema ISSN1672 6472 CN11 5180 Q DOI 10 13346 j mycosystema 200370 基金项目 中国医学科学院医学与健康科技创新工程 重大协同创新项目 2016 I2M 2 002 Supported by CAMS Initiative for Innovative Medicine 2016 I2M 2 002 Corresponding author E mail xkxing2009 ORCID LIU Na 0000 0002 2905 2248 XING Xiao Ke 0000 0002 1845 5076 Received 2020 11 25 accepted 2020 12 09 Copyright 2021 Institute of Microbiology CAS All rights reserved jwxt Http journals Tel 86 10 64807521 菌物学报 851 邢晓科 中国医学科学院药用植物研究所研究员 博士生导师 加拿大不列颠哥伦比亚 大学 UBC 比利时鲁汶大学 KU Leuven 访问学者 研究方向为药用植物菌根生 态学 近年来在菌根真菌与兰科药用植物的共存分化 地理分布 生态位形成及菌 根互作网络结构等方面进行了系列研究 主持完成国家自然科学基金项目 3 项 已 在 J Biogeogr Proc R Soc B Oikos Sci China Life Sci Mycorrhiza Mycologia Mycopathologia 菌物学报 等国内外期刊发表论文 50 余篇 菌根真菌提高植物抗旱性机制的研究回顾与展望 刘娜 赵泽宇 姜喜铃 邢晓科 中国医学科学院北京协和医学院药用植物研究所 北京 100193 摘 要 菌根真菌与全世界约 97 的维管植物具有广泛的共生关系 大量研究结果显示菌根植物相比于 非菌根植物对于干旱胁迫具有更高的耐受性 说明菌根真菌在植物抗旱过程中发挥着重要作用 本文对 近年来国内外在菌根真菌协助植物抵御干旱作用机制方面的研究进行了归纳和总结 主要包括在干旱胁 迫下菌根真菌对植物生理学特性的影响机制 菌根真菌提高植物抗旱性的分子机制以及菌根真菌对植物 次生代谢途径的影响机制等 3 个方面 当前菌根真菌增强植物抗旱性的生理机制方面的研究较为深入 而其他两个方面的研究则相对薄弱 随着分子生物学技术的发展 菌根真菌增强植物抗旱性的分子机制 和涉及的相关代谢通路将被进一步揭示 本文旨在呈现菌根真菌提高植物抗旱性机制的研究前沿 为菌 根互作更深层次的理论研究以及功能菌剂的研发提供一定的理论参考 关键词 丛枝菌根 外生菌根 杜鹃类菌根 兰科菌根 干旱 机制 引用本文 刘娜 赵泽宇 姜喜铃 邢晓科 2021 菌根真菌提高植物抗旱性机制的研究回顾与展望 菌物学报 40 4 851 872 Liu N Zhao ZY Jiang XL Xing XK 2021 Review and prospect of researches on the mechanisms of mycorrhizal fungi in improving plant drought resistance Mycosystema 40 4 851 872 刘娜 等 菌根真菌提高植物抗旱性机制的研究回顾与展望 Review 菌物学报 852 Review and prospect of researches on the mechanisms of mycorrhizal fungi in improving plant drought resistance LIU Na ZHAO Ze Yu JIANG Xi Ling XING Xiao Ke Institute of Medicinal Plant Development Peking Union Medical College Chinese Academy of Medical Sciences Beijing 100193 China Abstract Mycorrhizal fungi have an extensive symbiotic relationship with about 97 of vascular plants across the world Quantities of researches show that mycorrhizal plants own higher tolerance to drought than non mycorrhizal plants indicating that mycorrhizal fungi play an important role in plant drought resistance In this paper the mechanism of mycorrhizal fungi in assisting host plants to resist drought was summarized Three main aspects are included the effect of mycorrhizal fungi on physiological characteristics of plants under drought stress molecular mechanisms of mycorrhizal fungi in enhancing drought resistance of plants and influence of mycorrhizal fungi on plant secondary metabolic pathways Currently researches on physiological mechanisms of plant drought resistance under enhancement of mycorrhizal fungi are comparatively deepgoing while the researches of other two aspects are relatively weak With the development of molecular biology technology the molecular mechanism of mycorrhizal fungi enhancing plant drought resistance and related metabolic pathways will be further revealed Hopefully this paper provides a helpful reference to Chinese researchers in theoretical research of mycorrhizal interaction and development of functional drought resistant agent Key words arbuscular mycorrhiza ectomycorrhiza ericoid mycorrhiza orchid mycorrhiza drought mechanism 干旱是威胁药用植物和农作物生产的 一个重要因素 水分亏缺使植物萎蔫 生理 生化功能受损 导致农作物减产 药用植物 有效成分积累降低等 据统计 全世界约 97 的维管植物都能形成菌根 主要包括丛枝菌 根 AM 外生菌根 EcM 兰科菌根 OrM 和杜鹃类菌根 ErM 4 种类型 Brundrett 1 influences allocation of phosphate within the plant and phosphate starvation responses The Plant Cell 14 8 1751 1766 Wang JG Zheng R Bai SL Liu S Yan W 2012 Responses of ectomycorrhiza to drought stress Chinese Journal of Ecology 31 6 1571 1576 in Chinese Watts Williams SJ Cavagnaro TR Tyerman S 2019 Variable effects of arbuscular mycorrhizal fungal inoculation on physiological and molecular measures of root and stomatal conductance of diverse Medicago truncatula accessions Plant Cell Environment 42 1 285 294 Wang WX Zhang F Chen ZL Guo C He JD Zou YN Wu QS 2017 Responses of phytohormones and gas exchange to mycorrhizal colonization in trifoliate orange subjected to drought stress Archives of Agronomy and Soil Science 63 1 14 23 Wang XL Liu YM Gao RM 2019 Effects of ectomycorrhizal fungi on the growth of Pinus tabulaeformis seedlings under drought stress Science of Soil and Water Conservation 17 2 70 76 in Chinese Wang XX Hoffland E Feng G Kuyper TW 2017 Phosphate uptake from phytate due to hyphae mediated phytase activity by arbuscular mycorrhizal maize Frontiers in Plant Science 8 684 Wang Y Ding GJ 2012 Physiological responses of mycorrhizal Pinus massoniana seedlings to drought stress and drought resistance evaluation Chinese Journal of Applied Ecology 29 6 822 828 in Chinese 刘娜 等 菌根真菌提高植物抗旱性机制的研究回顾与展望 Review 菌物学报 870 Wang Y Wu WH 2013 Potassium transport and signaling in higher plants Annual Review of Plant Biology 64 1 451 476 Wang Y Zhang C Ding GJ Li ZQ 2019 Effect of water stress on physiology and photosynthesis properties of ectomycorrhizal Pinus massoniana seedlings Journal of Zhejiang Forestry Science and Technology 39 4 1 8 in Chinese Wei M Tong QY Chai RJ Ding JH 2018 Effects of orchid mycorrhizal fungi on growth antioxidant defense and related gene expression of Dendrobium officinale under drought stress Acta Botanica Boreali Occidentalia Sinica 38 10 1905 1912 in Chinese Wu HH Zou YN Rahman MM Ni QD Wu QS 2017 Mycorrhizas alter sucrose and proline metabolism in trifoliate orange exposed to drought stress Scientific Reports 7 42389 Wu QS He JD Srivastava AK Zou YN Ku a K 2019 Mycorrhiza enhances drought tolerance of citrus by altering root fatty acid compositions and their saturation levels Tree Physiology 39 7 1149 1158 Wu QS Srivastava AK Zou YN 2013 AMF induced tolerance to drought stress in citrus a review Scientia Horticulturae 164 77 87 Wu QS Zou YN Zhan TT Liu CY 2010 Polyamines participate in mycorrhizal and root development of citrus Citrus tangerina seedlings Notulae Botanicae Horti Agrobotanici Cluj Napoca 38 3 25 31 Xie X Huang W Liu FC Tang NW Liu Y Lin H Zhao B 2012 Functional analysis of the novel mycorrhiza specific phosphate transporter AsPT1 and PHT1 family from Astragalus sinicus during the arbuscular mycorrhizal symbiosis New Phytologist 198 3 836 852 Xiong XR Ouyang JH Kong HY Xiong D Ou J 2018 Effects of water stress on the ultra microstructure of root cells of Rhododendron annae mycorrhizal seedlings Acta Agriculturae Universitatis Jiangxiensis 40 5 965 971 in Chinese Xiong LM Zhu JK 2003 Regulation of abscisic acid biosynthesis Plant Physiology 133 1 29 36 Xu C Wu XQ 2016 Physiological and proteomic analysis of mycorrhizal Pinus massoniana inoculated with Lactarius insulsus under drought stress Russian Journal of Plant Physiology 63 5 709 717 Xu C Wu XQ 2009 Drought responses and related endogenous polyamine changes in mycorrhizaed Pinus massoniana Acta Botanica Boreali Occidentalia Sinica 29 2 296 301 in Chinese Xu C Wu XQ 2012 Drought resistance of Pinus massoniana seedlings inoculated with ectomycorrhizal fungi Journal of West China Forestry Science 41 6 43 47 in Chinese Xu L Naylor D Dong ZB Simmons T Pierroz G Hixson KK Kim Y Coleman Derr D 2018 Drought delays development of the sorghum root microbiome and enriches for monoderm bacteria Proceedings of the National Academy of Sciences 115 18 E4284 E4293 Xu LJ Hao ZP Xie W Li F Chen BD 2018 Transmembrane H and Ca 2 fluxes through extraradical hyphae of arbuscular mycorrhizal fungi in response to drought stress Chinese Journal of Plant Ecology 42 7 764 773 in Chinese Xu LX Han LB Huang B 2011 Membrane fatty acid composition and saturation levels associated with leaf dehydration tolerance and post drought rehydration in kentucky bluegrass Crop Science 51 1 273 281 Yooyongwech S Samphumphuang T Tisarum R Theerawitaya C Cha um S 2016 Arbuscular mycorrhizal fungi AMF improved water deficit tolerance in two different sweet potato genotypes involves osmotic adjustments via soluble sugar and free proline Scientia Horticulturae 198 107 117 Zhang F Zou YN Wu QS Ku a K 2020 Arbuscular Mycorrhizas modulate root polyamine 特约综述 22 April 2021 40 4 851 872 Mycosystema ISSN1672 6472 CN11 5180 Q 菌物学报 871 metabolism to enhance drought tolerance of trifoliate orange Environmental and Experimental Botany 171 103926 Zhang F Ni QD Zou YN Wu QS Huang YM 2017 Preliminary study on the mechanism of AMF in enhancing the drought tolerance of plants Journal of Fungal Research 15 1 8 13 in Chinese Zhang F Zou YN Wu QS 2019 Effects of Funneliformis mosseae on the expression of antioxidant enzyme genes in trifoliate orange exposed to drought stress Mycosystema 38 11 2043 2050 in Chinese Zhang HQ Wei SZ Hu WT Xiao LM Tang M 2017 Arbuscular mycorrhizal fungus Rhizophagus irregularis increased potassium content and expression of genes encoding potassium channels in Lycium barbarum Frontiers in Plant Science 8 440 Zhang JH Jia WS Yang JC Ismail AM 2006 Role of ABA in integrating plant responses to drought and salt stresses Field Crops Research 97 1 111 119 Zhang ZF Zhang JC Xu GP Zhou LW Li YQ 2019 Arbuscular mycorrhizal fungi improve the growth and drought tolerance of Zenia insignis seedlings under drought stress New Forests 50 4 593 604 Zhang ZF Zhang JC Huang YQ Yang H Luo YJ Luo AY 2013 Effects of arbuscular mycorrhizal fungi on plant drought tolerance research progress Chinese Journal of Ecology 32 6 1607 1612 in Chinese Zhang WQ Yan W 2013 Effect of ectomycorrhizae on the growth of Pinus sylvestris var mongolica seedlings Acta Botanica Boreali Occidentalia Sinica 33 5 998 1003 in Chinese Zhao M Ning XZ Xie XQ Yan SQ Wang JG Bai SL 2019 Molecular mechanisms of Prunus mongolica mycorrhizal seedlings responding to drought stress Journal of Northwest A F University Natural Science Edition 47 8 90 99 in Chinese Zou JJ Li XD Ratnasekera D Wang C Liu WX Song LF Zhang WZ Wu WH 2015 Arabidopsis calcium dependent protein kinase8 and catalase3 function in abscisic acid mediated signaling and H 2 O 2 homeostasis in stomatal guard cells under drought stress The Plant Cell 27 5 1445 1460 附中文参考文献 陈连庆 王小明 裴致达 2010 石斛气生的兰科 菌根组织结构及其对御旱研究 生态环境学 报 19 1 160 164 何斐 2016 黄土高原丛枝菌根真菌 AMF 提高刺 槐抗旱性机制 西北农林科技大学博士论文 西安 95 96 李涛 杜娟 郝志鹏 张莘 陈保冬 2012 丛枝 菌根提高宿主植物抗旱性分子机制研究进展 生态学报 32 22 7169 7176 梁建萍 贾小云 刘亚令 吴云 周然 冯前进 2016 干旱胁迫对蒙古黄芪生长及根部次生代谢物含量 的影响 生态学报 2016 14 4415 4422 陆兴杨 2019 外生菌根真菌提高马尾松幼苗的抗 旱性研究进展 安徽农学通报 25 8 25 28 30 马坤 王彦淇 杨建军 李璐 王颖 马焕成 2017 不同干旱胁迫条件下丛枝菌根真菌对木棉叶 绿素荧光参数的影响 植物资源与环境学报 26 3 35 43 王琚钢 峥嵘 白淑兰 刘声 闫伟 2012 外生 菌根对干旱胁迫的响应 生态学杂志 31 6 1571 1576 王秀丽 柳昱旻 高润梅 2019 外生菌根真菌对 干旱条件下油松幼苗生长的影响 中国水土保 持科学 17 2 70 76 王艺 丁贵杰 2012 干旱胁迫下外生菌根真菌对 马尾松幼苗生长和微量元素吸收的影响 浙江 农林大学学报 29 6 822 828 王艺 张弛 丁贵杰 黎之骞 2019 干旱胁迫对 马尾松菌根化苗木水分生理和光合特性的影 响 浙江林业科技 39 4 1 8 魏明 童秦怡 柴瑞娟 丁佳红 2018 兰科菌根 真菌对干旱胁迫下铁皮石斛生长和抗氧化能 力及相关基因表达的影响 西北植物学报 38 10 1905 1912 刘娜 等 菌根真菌提高植物抗旱性机制的研究回顾与展望 Review 菌物学报 872 熊贤荣 欧阳嘉晖 孔海燕 熊丹 欧静 2018 水 分胁迫对桃叶杜鹃菌根苗根系超微结构的影 响 江西农业大学学报 40 5 965 971 徐超 吴小芹 2009 菌根化马尾松对干旱胁迫的 响应及其内源多胺的变化 西北植物学报 29 2 296 301 徐超 吴小芹 2012 菌根化马尾松植株抗旱性研 究 西部林业科学 41 6 43 47 徐丽娇 郝志鹏 谢伟 李芳 陈保冬 2018 丛 枝菌根真菌根外菌丝跨膜 H 和 Ca 2 流对干旱胁 迫的响应 植物生态学报 42 7 764 773 张菲 倪秋丹 邹英宁 吴强盛 黄咏明 2017 AMF 增强枳抗旱性作用机制的初步研究 菌物研 究 15 1 8 13 张菲 邹英宁 吴强盛 2019 AM 真菌摩西管柄 囊霉对干旱胁迫下枳抗氧化酶基因表达的影 响 菌物学报 38 11 2043 2050 张文泉 闫伟 2013 外生菌根菌对樟子松苗木生 长的影响 西北植物学报 33 5 0998 1003 张中峰 张金池 黄玉清 杨慧 罗亚进 罗艾滢 2013 丛枝菌根真菌对植物耐旱性的影响研究 进展 生态学杂志 32 6 1607 1612 赵敏 宁心哲 谢旭强 闫三强 王琚钢 白淑兰 2019 蒙古扁桃菌根苗对干旱胁迫的分子响应 机制 西北农林科技大学学报 自然科学版 47 8 90 99 本文责编 韩丽