海水胁迫对菠菜(Spinacia olerancea L.)叶绿体活性氧和叶绿素代谢的影响

»29 »8 ù2009 M83 ÿÐACTAECOLOGICASINICAVol.29,No.8Aug., 2009htp//Á[ “SEÚnp VÄ SùÁ ù[ “20050307031; Ê 81 – SÐÁ ù[ “BK2006140là ° ù2008-05-09; ©è ° ù2008-12-10＊YßT€Correspondingauthor.E-Z £} çk’SpinaciaoleranceaL. s8Ÿ„ s Í}†¥•Y孙 锦1, 2,贾永霞1,郭世荣1,＊,李 娟11. 2Ø jvÐó\Ðý, 2Ø 210095;2.Å Õ 8 j SÐý v’ùî î, §Ü 730070K1[Z £ ùË ÖÕõk’„ 0Z £ ÖÕ€ §3| ¹ k‹,¨ £ ¡ZE,ùî Z £} çk’SpinaciaoleranceaL. s8ŸROS„ s ÍChl}†¥•Y。²TV ü,Z £} ç/, 2ñk’ ÖÕ s8 Ñ| Ö0O-·2 Á 3 Îq、VÄ _H2O2„d „MDAc A÷ 6Ú,i Oõk’¥4Ú–v¿€ §3| ;õk’ s8 ¥ÑÄþ Ä ½SOD、 FÊÓ ÖVÄþ ½APX、‡ÅtÎð ½GRŸ„ FÊÓ ÖAsA、Îð˜‡ÅtGSHc  ®¿€ §3| ,7VÄþ ½PODŸÚ¿€ §3| ;;Ä4J,ÚMVÉB„ P2ñk’ ÖÕ s8 O-·2Á 3Î qF y、H2O2c 4Ú、 ÉVÄF,Ÿ b“4AsA üA†® k’ s8 ROS £ Ü,ϳ ®MV/î¥ø¥ ÉVÄ ]。Z £} ç/, 2ñk’ ÖÕ Ë s ÍbChlb、 s ÍaChla †î -8þÉð s Í ÖPchl、 ÃðúⅨMg-protoIX、ðúⅨProtoⅨ„ UúðⅢUroⅢc  üA†®,7‰ ä ÍðPBG„δ-¼[û ÖALAÆ,Chl†î sžE¸,i Oõk’¥ sEñv¿€ §3| ;MVÉB„F  ƒÕ sEñ,7AsA†sϳ ®Z £} ç„MV/î¥E¸T¨。Z £} ç üA4Ú õk’ Ë s Í ½ChlaseŸ7€ §3| Àµ•Y,MV Øõk’ChlaseŸ¥•Yñv¿€ §3| ,ŒAsA2ñ ÖÕ ËChlaseŸ Àµ üA•Y。  ²T ª ü,Z £}ç/,k’ s8 ROSÐChl}† á MM1,‚ÇYV s8 ¥Ä ] PChl†³,7 O PChl†î¥PBG_UroⅢÄ„ç sE。 0Z £ ÖÕ€ §3|  s8 b“ROSö1YVSOD„AsA-GSHÖÌ“d, b“ ï ,h ROS s8 ¥Ä å „Chl†î¥ sEñ,i OZ £} ç ChlaseŸ¥•Y l;7Z £ ùË ÖÕõk’ s8 b“ROSö1G ¿SOD„POD,ROS¥ b“ ïµK,V7Á ROSv  Æ, s8 ¥Ä å „Chl†î¥ sEñø,i OZ £} çA÷4Ú ChlaseŸ,F  Chl†³。1oMZ £;k’SpinaciaoleranceaL.; s8;Ÿ; s ÍÓcI|1000-0933200908-4361-11 Ïms Ë|Q143, Q945, Q948 ÓDS M ’AEffectsofseawaterstressonmetabolismofreactiveoxygenspeciesandchlorophylinchloroplastsofspinachSpinaciaoleranceaL.SUNJin1, 2, JIAYong-Xia1, GUOShi-Rong1,＊, LIJuan11ColegeofHorticulture, NanjingAgriculturalUniversity, Nanjing210095, China2VegetableInstitute, GansuAcademyofAgriculturalSciences, Lanzhou730070, ChinaActaEcologicaSinica, 2009, 2984361～ 4371.AbstractTheefectsofseawaterstresonmetabolismofreactiveoxygenspeciesROSandchlorophylChlinchloroplastoftwospinachcultivars, YuanyespinachseawatersensitivecultivarandHelanNo.3 seawatertolerantcultivarwereinvestigatedbyhydroponics.TheresultsshowedthatproductionrateofsuperoxideradicalO-·2, contentofhydrogenperoxideH2O2andmalonaldehydeMDAinchloroplastoftwospinachcultivarswereremarkablyincreasedbyseawaterwithhigherrateincv.Yuanye;Underthestresofseawater, theactivityofanti-oxidationenzymesuchassuperoxidedismutaseSOD, ascorbicacidperoxidaseAPXandglutathionereductaseGR, andthecontentofhtp//antioxidantsuchasascorbicacidAsAandglutathionereducedGSHinchloroplastofcv.Yuanyewerelowerthanthoseofcv.HelanNo.3, whileperoxidasePODactivityinchloroplastofcv.Yuanyewashigherthanthatofcv.HelanNo.3;ThesupplementofmethylviologenMV, thephoto-oxidant, enhancedtheproductionrateofO-·2, thecontentofH2O2 andMDAinchloroplastoftwospinachcultivars, whiletheadditionofAsA, theeliminatorforROS, decreasedROSlevelandaleviatedoxidizationofplasmolemma.ThecontentofchlorophylbChlb, chlorophylaChlaandprecursorofChlsuchasprotochlorophylPchl, Mg-protoporphyrinⅨ Mg-ProtoIX, protoporphyrinⅨ ProtoⅨ anduroorphyrinogenⅢ UroⅢwasremarkablydecreased, butthecontentofporphobilinogenPBGandδ-aminolevulinicacidALAwasincreasedbythestressofseawater, whichleadtoinhibitionforChlsynthesis, andtheinhibitionwasaggravatedbyMVandeliminatedbyAsA.ChlorophylaseChlaseactivityintheleavesofcv.YuanyewasimprovedwhileitwasnotinfluencedintheleavesofHelanNo.3underseawaterstress.TheChlaseactivityintheleavesofcv.YuanyewasmoregreatlyafectedbyMVtreatmentthanthatofHelanNo.3, however, ChlaseactivityoftwocultivarswasnotinfluencedbyAsA.TheseresultssuggestthatROSiscloselyrelatedtoChlmetabolism, whichnotonlyinjureplasmolemmabutalsoinhibittheprocesoftransationofPBGtoUroⅢ asaresultofChldecomposition.Inseawatertolerantcultivarcv.HelanNo.3, theROSeliminationismainlydependedonSODandAsA-GSHsystem, whichcouldaleviateoxidationinjuryofROStochloroplastmembraneandinhibitionofChlsynthesis, andChlaseactivitywaslessinfluencedbyseawaterstress;whileinseawatersensitivecultivarcv.Yuanye, itismainlydependedonSODandPOD, whichhaslimitedabilitytoeliminateROSandleadtoaccumulatemassROStosevereoxidationinjuryofchloroplastmembraneandinhibitionofChlsynthesis, andChlaseactivitywassignificantlyimprovedbyseawaterstress, whichenhancedChldecomposition.KeyWordsseawater;spinachSpinaciaoleranceaL.;chloroplast;reactiveoxygenspecies;chlorophyl s ÍChl ¹ o K1¥;† ä Í,‚Ç V[ lv ; ,7 OÎî;“d;“dQ‹Ï[ 1] 。±þ8 Chlc ¥Ú® | ¿Chl†îІ³¥î ÿ Ü™[ 2] 。Chl 3þ†î[3]¥ © B„„çCp¸,ûö•YChl¥†î,V7„ Chlc †®;Chl†³ “ ½\†³„;Ć³ ÕQ‹ ˘, Ï s Í ½ChlaseÐChl†³¥1“ á M[ 4] ;ŸROS9 V„ Chl†³[ 5],Yuzo©[ 6] ª¹ROS V •Ð Chl4- 6Ì¥ åÊ,V7F ÎChl†³。±þ®Ñ[ 7] 、SO2[8] 、NO2[ 9]、 £s} ç[5] 、 š º[10]©Hq/,Chl†³ÐROS}†µ1;÷} ç/,ROS Ž•ÐChl†³„E]Chl†î[E]ÊÄû‚ ü 。 s8 Á 3ROS¥1 [11],÷} ç/ s8 ROSv  Æ[ 12, 13] , s8  3VÄQ‹,;† ä ͆³,•Y ±þ;†T¨。Z £} ç/,k’SpinaciaoleranceaL. 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0.753a 15.613a15.079a11.346d10.825ef2.483b 1.546eCKMVAsA 0.157c 0.301c 0.467cd0.772b 0.301c 0.470b 0.192c 0.245c 0.488c 0.553cd 6.593d 9.409d11.219d11.484de3.031ab 4.948bT 0.165c 0.296c 0.482c 0.727bc0.265d 0.436c 0.141d 0.241c 0.365e 0.578c 10.022c12.977b13.633bc13.600c1.523c 1.298dTMV 0.106e 0.256d 0.322f 0.679d 0.209e 0.303f 0.118f 0.211d 0.316f 0.467e 4.321e 7.220e15.