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µ 2 jÛ\1 x ë 2é2 ®2ïC¿1,2* 1.µ 2vÐùî 3ý H Üsýµ 2 É ì 410125 2.µ 2 8 vùî îµ 2 É ì 410125 摘 要 æ¨ Illumina MiSeq 2500©½ Ü û 2ñ 1“ SJ11–3 a06g19–1–1–1 1ñ¹Z ÖÕ b Ö Õ± -ªôr ¥± 3þÉ©½ i²M1 3þÐsr 16S rRNAy V3V4 u ITS1u¥¶ a “· [± 3þ ² b²TV ü Õ± -ªr [M Ê ÖÆ Ê¹ª ] 0 5 Ê a0 ʤ ʹª ] Õ± -ªr Ï¥ Õ Ë ÷F¶ “Äñ÷Ú Õ± ªr a ¥M ²û 3 MÄr Ace aChao Shannon· ûµ î9F9sY¹ 25.2625.49 a24.5124.87 8.289.557 ¥ Shannon · µ]ñ® Simpson· 9v9 78.45275.69 Õ±ªr Ï ¥ Ts Ëí OTU Ë9µ î¿M OTU ñ Õ± - üA 6Ú9ÿ 28.73r ϵ¥ OTU Ë üAh OTUñ MÄ ËMÄ1 vhð ¥Mv9F b 关 键 词 ôr ± 3þ “ 中图分类号 S641.3； Q938.13 文献标志码 A 文章编号 1007−1032201802−0151−06 Effects of pepper cultivation on the microbial diversity of rhizosphere soil KANG Linyu1 LIU Zhoubin1 OU Lijun2 TONG Hui2 PENG Ying2 YUAN Zuhua1,2* 1.Longping Branch, Graduate School of Hunan University, Changsha, Hunan 410125, China; 2.Vegetable Institution, Hunan Academy of Agricultural Science, Changsha, Hunan 410125, China Abstract Two pepper inbred lines, ‘SJ11–3’, ‘06g19–1–1–1’, and a local variety ‘Zhangshu gang’ were used as materials, also using the Illumina MiSeq 2500 sequencing plat to sequencing the 16S rRNA gene V3 V4 regions of bacteria and ITS1 region of fungal, in the rhizosphere soil before and after pepper planting. And combining the bioinatics to analyze the richness, diversity index and community structure of the soil microorganism. The results showed that both Proteobacteria and Acidobacteria were the dominant bacteria in the soil before and after pepper cultivation. Ascomycota, Basidiomycotina and Fusarium were the dominant fungi. The bacteria species in the soil before and after the planting were more abundant and more diversified than the fungi. The relative abundance and community structure of soil bacteria and fungi had changed after pepper planting. The Ace, Chao and Shannon index of soil bacteria was increased,25.26 – 25.49, 24.51 – 24.87 and 8.28 – 24.87 respectively after pepper planting. The Simpson index of fungi was increased by 78.45 – 78.45, also Shannon index had a different degree of reduce after pepper planting. The number and types of OTU in soil were also changed after pepper planting, the specific OTU type in soil was reduced, while the number of bacteria OTU was significantly increased 28.73 after pepper planting. The effect of variety difference on OTU type of fungi was greater than that of bacteria, and the relative abundance of pathogenic fungi was significantly increased. Keywords pepper; rhizosphere soil; microbial diversity 收稿日期 2017–06–23 修回日期 2017–09–13 基金项目 SE S/¦ê9Ã[ “ 2014BAD05B04 作者简介 B Ö 1993 i oµmË ¦ « Vùî 3ö1V Y± 3þ aº8бþºTùî *YTïC¿ùîôö1V Y v ¡ 3 Ø 3Äs0 3þÐùî 152 µ 2 jØr V “ ”_ “ ”r Ä b kÏ Õ± -ªr Ï Ace aChao Shannon · A÷Ú¿ ¥· 7 Simpson · 5A÷®¿ bV ü Õ± -ªr Ï þÕ ÷F¶ “Äñ÷Ú ¿r B¥ “ ”r b] H Õ±ª ¶A÷ 6ÚþÕ¥ “9µ î9F7 ¶í üAMÄ¥ f/ þÕ¥ “µ î® b Ð CARTWRIGHT©[13] FIERER ©[14]ª¹r ± 3þ “бþ “ïM1¥²TBÁ b/îÕMÄ¥ðy V ®¿Õ± -r í±ÂÕ± ª ¥Â± 3þ Á 3 Y b kÏr ¥ OTU “Ð Ace Chao · MÄ p BÁ ¥ OTU Ë i µs µ¥OTU Ë Ù ]9 OTU Ë¥ vsÕ±-r Ïcµ µ¥ OTU Ë7Õ± ªr µ¥ OTU Ë üAh O] ÖÕW OTU Ë¥µs {v¿ bV ü Õ±ªr ϱ 3þ¥ Ë Fî 3 üA¿M bÕMÄ V ] ÖÕ 3ÉâVñÏÁ 3¥ô“s ßþr ± 3þ¥YÁ¥[15–16]9V r ± 3þYV¿Mô“s ßþ¥îs W¤ eÅô ð± 3þ¥Õ Ë [17–18] bÕ±ªô“s ßþ¥µsÁr W¥M Üu å/î ñ ñ ¥ v4Ú[19–20] b kÏ Õ± ª ¾ aö¹ ´ ©±þhð ¥v9F b - ¦¥ùî[21]9V ü ¾ ö¹ h]¥ª ]hð 7ª ]hð 8v 9 öøKÅ îÕ±þ¥ 3ÉâÁ±éhÁ .À µ§ yNÕ± Ù ö Pr »û þÕ÷F¶¥ “ ”r ]Hö/î þÕ “h Ov9F±þhð ¥M b 参考文献 [1] ¬k Ù Wy©Z 2Pór ± 3þÐr y0¥1“ [J] £{TþÐ 2010 314 530–535. 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