亚精胺对黄瓜幼苗灰霉病的诱抗作用

ðÚÂÛ¼ ìç ¾h¥º FT¨＊尚庆茂＊＊ 张志刚ÏS jYÈ20[ ,ø H Vr50[ 11] .ç ¾h 7öW,“ S ™Û“,Î VϙI N、 N0、 X’、ñ’、7ò、’ø、 ÚT、1©‹¨ 3 ÿÐ 2008 M4 »19  »4 ù ChineseJournalofAppliedEcology, Apr.2008, 194825-830DOI 10.13287/j.1001-9332.2008.0211200Õ±þ¥½、T L s äF®[ 12] . “ -,Ûç ¾h[ÄÐ j0]͹ö, ÛÄÐ j0 P¨Q ”、i¥‚9F,hð FŸ9 ,]ÍrT9 Û­†®,ÎöÁ j0— „Ìâê ™,²] ¦ ËrB. k[ÛCucumissativusL.¹‹ ,ùîSpdç ¾h S ™/Û¼ ì¥h f· ”、]Þ ½Ÿ FhM1þÉc ¥•Y,[ ü ’SpdºÛ¼ ì Fç ¾h¥rT  V ¥ 3 ØÅ,¹ æ¨Äк F4Ï³ç ¾hp¸4 Ø ‚„LlG .1 ‹ ÐZE1.1 k‹ Û ÖÕʨÃù4| .Spd¹SIGMA³Á ÖÁ Ö} ’S2626,í äA8, á0.925 g·L-1,B≥98, ’ |Spd2.206 ml,¨• £ç ¸À1 000ml ¥î2 000 mg·L-1 A,¨.ç ¾h ®Š S/ SÐý± 3þ i4.k¿2005 MÏS j SÐý v’½ñùî îj ðÑ i É›.Õ0ÑÏÕ4h,bè24h,315 °Ê4èœ BÁ¥Õ0l Æ Ñ n9 cm9 cmÏ,¨¤ F¯†É[ 13] .ÑiÌâHq¹æ23±3℃/12±2℃,1 –;v „;Û ù, Ü M A75～95.1.2 º Øç k ,Spd 0v、50、100、150、200、250 mg·L-1“  i. Û uF 9, 3Q¯, Ä Øl u20é.46 °Û¼ ì»2ˇZ H,¨ÿ  |Spdÿ ÆÉ öÔÿ2 cmÉ›ô Sº, Äé¨ ¹20 ml,Ø HSpd ·Aà 1n“;2dª ë ¨ ɛĺ, Ä Øl u¨  ¹60ml, ë、  ¨ ÒÀ ·A¸¿ 댂/¯.v¨© • £}9.1.3 h ¤Õ410 °,  Sºª4d¤Õç ¾h .sY¨ f„¹0ÇœA ÕZE¤Õ.f¤Õ¨u d u |°å¹0.4cm¥ f¤Õ¿»2 ˇž ë, Ä ËP· öò¤Õ1ñ f.¨¿©çhê°å,9 Øh f· ”.¹0ÇœA¤Õ ¥Å¹0ÇœA i¹4.3105ñ·ml-1.¨ ¸  9 |¹0ÇœA,|±é»2 ˇ› †Ï À¿¹0ÇœAÏÿ5 s, TVñÏ]¸¹0ÇœAÐ  ð ˤ.¨¿¼ ì™ ÿ·S 3 Ø 3Ä·S©ç.f„¹0ÇœA¤Õ] HÉ›.¤Õ›8ªì5­Â{©É› Ä5 Ø,æ21±2℃/15±1℃, b  A90[ .1.4 ©çZE1.4.1h f· ” sY¿¤Õª2、4、6 d¨ E3Œ­E©çhê˜å、å,  Ü ´T¹hê°å,h f· ”9 Ø T Â/h f· ” ∑ Ø òhê°å Ø ¥hê ” îµ ØÏKvhê°å1001.4.2 3 Ø 3Ä·S sY¿ºª3、6、9、12„15 dg |¼ ì»2 ˇɛ 3 Ø 3Ä·S©ç.•vMoerschbacher©[ 14]ZE©çPAL、PODŸ;•vMiler©[15]ZE©çPPOŸ;•vë¡o©[ 16]ZE©ç9p、 ËÛ[c ;•vMorison[ 17]ZE©ç É Íc .1.4.3™ ÿ·S 426 °,  Sºª20d,Ø°¼ ìéÚ、ÔY、 Ë ”、 ë、 †é7É „ †éÄÉ . ì· ”ÔY/éÚ †éÄÉ 100.îµ ” ¨SAS ÈqDuncan¯µEÉ›1 µsA÷Ÿs,mV ” ¹3Q¯¥ Ü ´.2 ²TÐs2.1 “÷SpdÛ¼ ìh f· ”¥•Y®m1 V©,ÐvM1,“÷Spd üA†® m1 “÷SpdÛ¼ ìh f· ”¥•YFig.1 Effectsofexogenousspermidineondiseaseindexincu-cumberseedlings.Ⅰ0mg·L-1Spd;Ⅱ50mg·L-1 Spd;Ⅲ100 mg·L-1 Spd;Ⅳ150mg·L-1 Spd;Ⅴ200mg·L-1 Spd;Ⅵ250 mg·L-1Spd.826 ‹ ¨ 3 ÿ Ð  19 Û¼ ì¥h f· ”.‚]©ç H ù¤Õª2、4、6d,Spd iÐh f· ”ïCM]¥MÄ p, ÛSpd iïv 6Ú,h f· ”ïv†®,†®žBçñª,» Û“Spd i¥ 6Ú79F. ª üSpd†®Û¼ ìh f· ”i aR¥ Ø i,[150mg·L-1 ØK®,¤Õª2、4、6 dh f· ”sY1v†® 10.0、18.5„25.4.2.2 “÷Spdç ¾h S ™/Û Ë]Þ ½Ÿ¥•Y®V1 V©, Û Sºª HW¥üÉ,ò Ø¥ Sºª6、9 d,PODŸ ÛSpd i¥ 6Úï5†®ª 6Ú¥MÄ t ]; Sºª15 d, ÛSpd i¥ 6Ú,PODŸï5 6Úª†®¥MÄ t ].‚]©ç H ù,Spdò i Ø¥PPOŸÚ¿v,SpdPPOŸ¥9 9VCBç¥ ir‹. Sºª3、6 d,Spd i®¿150mg·L-1 H, PPOŸ ÛSpd i¥ 6Ú79 ;Spd iÚ¿150 mg·L-1 H,PPOŸ ÛSpd i¥ 6Ú7h Ð;Spd i¹150 mg·L-1 H,PPOŸrKv´,sY1v4Ú 15.5„27.5. Sºª9、12、15 d,PPOŸÚ‚C200mg·L-1 Ø,sY1v9F 38.9、22.6„29.7.2.3 “÷Spdç ¾h S ™/Û ËÏ FhM1þÉc ¥•Y®V2 V©, Û Sºª HW¥üÉ,òØ9pc ï5†®ª 6Ú –ª û Ú £ Ü¥MÄ t ].¤Õç ¾h - Pºª3 d,Spdòi Ø¥9pc  Ú¿v,9pc  ÛSpdi¥ 6Ú79F,Spd i¹200mg·L-1 HrKv´,1v9F13.7;¤Õç ¾h ª Pºª6、9、12、15 d,Spdò i Ø¥9pc ïv 6Ú,°ÀÚ¿v.V1 “÷Spdç ¾h S ™/Û¼ ì ËÏ]Þ ½Ÿ¥•YTab.1 EffectsofexogenousspermidineonactivitiesofdefenseenzymesincucumberseedlingsleavesunderinfectionofB.cinereaSpd iSpermdineconcentrationmg·L-1 Sºª9 d,Spdò i Ø¥ ËÛ[c ÐvíA÷µs; Sºª12、15 d,Spdò i Ø¥ ËÛ[c  ®¿v.‚]©ç H ù,Spdò i Ø¥ É Íc Ú¿v,Spd É Í†î¥\ÉT¨VCBç¥ ir‹. Sºª3、6、9 d,Spd i®¿150 mg·L-1 H, É Íc  ÛSpd i¥ 6Ú79F,Spd iÚ¿150 mg·L-1 H, É Íc ÛSpd i¥ 6Ú7h ,Spd i¹150 mg·L-1 H, É Íc rKv´,sY1v4Ú 17.8、52.0„23.6; Sºª12、15 d, É Íc ‚´C200 mg·L-1 Ø,sY1v9F18.3„44.5.2.4 “÷Spdç ¾h S ™/Û¼ ì 3É⥕Y®V3 V©,“÷Spdç ¾h S ™/Û¼ ì¥ÔY、éÚ、9 ë、 †é7É 、 †éÄÉ、 ì· ”6[·S µ üA¥\ÉT¨;Z 7 ”9µ\ÉT¨,ŒÐvM1µs‚A÷.SpdÛ¼ ì 3É¥\ÉT¨VC üA¥ ir‹, Û i¥ïv 6Ú,Spd¼ ì¥\ÉT¨ïv9 ,ò[·SrKv´ª, ÛSpd i¥à 6Ú,\ÉT¨ïvh Ð.100 mg·L-1Spd ØÛ¼ ì 3É¥\ÉT¨Kv,ÔY、9 ë、 †éÄÉ 、 ì· ”sY1v4Ú 8.3、35.2、49.4„32.5.3  ‚“ -,1¿“÷Spdº±þ FhŸ[\ɱé 3É¥ùî7n¡.bÆ‚©[ 18]ùîV ü,d IâHq/õ¤ ¨“÷Spd Vrž ìrT. k²TV ü,“÷Spd VºÛ¼ ìç¾h¥ FŸ,h f· ”1vKÚ†®25.4;\É ç ¾h S ™/Û¼ ì¥ 3Éâ,ÐvM1, ì· ”KÚ9F32.5.PAL、POD、PPO ±þ Fh}†VñÏ¥1o½. ñ ÌŸ¥Ú®Ð±þ FhQ‹µ“ Es á M¥1“,YȨT™ ±þ8 ]ÏQ‹¥1·S.PAL  6BZ ë, ñ» ÐXµ¥]Þ ½s0²†,•Y ²,V7Ø« ½Ÿ. ùî²TV ü,“÷Spd تۼ ì ËÏPAL、POD、PPOŸïC‚]¥MÄ t ], Û Sº HW¥üÉ,PALŸïv†®,PODŸïv9 ,PPOŸï5†®ª 6Ú†®¥MÄ t ].ƒÕMÄ t ]Ðv HW  ûBÁ,ŒSpd Ø¥3Õ ½Ÿœñ k ùWB°Ú¿v £ Ü,PAL、POD、PPO3Õ]Þ ½ŸKÚsY1v9F48.4、635.9„38.9.yN,ƒt ½ŸÐ“÷SpdºÛ¼ ì Fç ¾hµBç¥M1Ÿ,Û V YV4Ú828 ‹ ¨ 3 ÿ Ð  19 ƒt ½¥Ÿ9 ·Þhð S Æ、 ¢ü¥ ï.p ËþÉ }†Áþ ±þ .¥ Fhy0, “·Þhðþ S ™¥T¨, ª¹ ±þ FhÅ­B[ 22].9p„ ËÛ[û p ËþÉ, }†ÐPAL、POD、PPO á MM1. 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