资源描述：
( h FT尚庆茂 张志刚(S jY20% , H Vr50% 11 . h )7W,“ S (, VI N、 N0、 X、7、 T、1 3 2008 M4 19 4 ChineseJournalofAppliedEcology, Apr.2008, 19(4):825-830DOI :10.13287/j.1001-9332.2008.0211200、T L# s F 12 . “ -,( h j0, j0 PQ 、i9F,h ) F9 ,rT9 , j0 = , rB. k(CucumissativusL.) ,Spd h ) S /( h f 、 # FhM1c Y, Spd( F hrT# V ? 3 , F4 hp4 LlG .1 ZE1.1 k ( 4|.SpdSIGMA ( S2626, A8, 0.925 gL-1,B98%), |Spd2.206 ml, 1 000ml 2 000 mgL-1 A,!. h ) S/ =S 3 i4.k2005 MS j S v j i =.04h,b24h,315 4? B0l !n(9 cm9 cm), F 13 .iHq:(233)/(122),1 ;v ; , (M A75%95%.1.2 ) ! k$ ,Spd !0(v)、50、100、150、200、250 mgL-1“ i. uF !9, 3Q, ) l u20.46 ( 2=Z H, |Spd ( 2 cm)= S, 20 ml,) HSpd A 1!n;2d= , ) l u (60ml,= 、= ( A= /.v 9.1.3 h )410 , S4d h ).sY )f0A ZE.)f:u d u |0.4cm )f2 = , =P =1 )f.h,9 h f .0A: 0A i4.3105ml-1. 9 |0A,|2 = 0A5 s, TV0A = (. S# 3 3S.)f0A H.85 5 ,(212)/(151), b A90% .1.4 ZE1.4.1h f sY2、4、6 d E3Eh、:, (Th,h f 9 T /:h f = () =h) =h ) Kvh100%1.4.2 3 3S sY3、6、9、1215 dg | 2 = 3 3S.vMoerschbacher 14ZEPAL、POD;vMiler15ZEPPO;vo 16ZE9p、 c ;vMorison 17ZE ! c .1.4.3 S 426 , S20d, 、Y、= 、= 、 7 . =(Y/) 100. (SAS qDuncanE1 #sAs,mV (3Q (.2 Ts2.1 Spd( h f Ym1 V,vM1,Spd A m1 Spd( h f YFig.1 Effectsofexogenousspermidineondiseaseindexincu-cumberseedlings.:0mgL-1Spd;:50mgL-1 Spd;:100 mgL-1 Spd;:150mgL-1 Spd;:200mgL-1 Spd;:250 mgL-1Spd.826 3 19 ( h f . H (2、4、6d),Spd ih f CMM? p, Spd iv 6,h f v,B, “Spd i 679F. Spd( h f i aR) i,150mgL-1) K,2、4、6 dh f sY1v 10.0%、18.5%25.4%.2.2 Spd h ) S /(= YV1 V, S HW,) S6、9 d,POD Spd i 65 6M t ; S15 d, Spd i 6,POD5 6M t . H ,Spd i) PPO(v,SpdPPO9 9VCB ir. S3、6 d,Spd i150mgL-1 H, PPO Spd i 679 ;Spd i150 mgL-1 H,PPO Spd i 67h ;Spd i150 mgL-1 H,PPOrKv,sY1v4 15.5%27.5%. S9、12、15 d,PPOC200mgL-1) ,sY1v9F 38.9%、22.6%29.7%.2.3 Spd h ) S /(= FhM1c YV2 V, S HW,)9pc 5 6 M t . h ) -( P3 d),Spdi) 9pc (v,9pc Spdi 679F,Spd i200mgL-1 HrKv,1v9F13.7%; h )( P6、9、12、15 d),Spd i) 9pc v 6,v.V1 Spd h ) S /( = YTab.1 EffectsofexogenousspermidineonactivitiesofdefenseenzymesincucumberseedlingsleavesunderinfectionofB.cinereaSpd iSpermdineconcentration(mgL-1) S9 d,Spd i) c vAs; S12、15 d,Spd i) c (v. H ,Spd i) ! c (v,Spd ! TVCB ir. S3、6、9 d,Spd i150 mgL-1 H, ! c Spd i 679F,Spd i150 mgL-1 H, ! c Spd i 67h ,Spd i150 mgL-1 H, ! c rKv,sY1v4 17.8%、52.0%23.6%; S12、15 d, ! c C200 mgL-1) ,sY1v9F18.3%44.5%.2.4 Spd h ) S /( 3?YV3 V,Spd h ) S /( Y、9= 、 7 、 、 6S ( AT;Z 7= 9T,vM1sA.Spd( 3TVC A ir, iv 6,Spd Tv9 ,SrKv, Spd i? 6,Tvh .100 mgL-1Spd) ( 3TKv,Y、9= 、 、 sY1v4 8.3%、35.2%、49.4%32.5%.3 ) “ -,1Spd Fh# 37n.b 18V ,d IHq/ Spd Vr rT. kTV ,Spd V( h F,h f 1vK25.4%; h ) S /( 3?,vM1, K9F32.5%.PAL、POD、PPO FhV1o. FhQ“ Es M1“,Y$T 8 =Q1S.PAL 6BZ , ?X s0,Y ,V7 .TV ,Spd) ( = PAL、POD、PPOCM t , S HW,PALv,PODv9 ,PPO5 6M t .M t v HW B,Spd) 3 k WBv ,PAL、POD、PPO3 KsY1v9F48.4%、635.9%38.9%.yN,t Spd( F hBM1,( V ?YV4828 3 19 t 9 h ) S 、 ? .p # . Fhy0, “h S T,$ FhB 22.9p p , PAL、POD、PPO MM1. ! %C1sB,h 3 S pT,7 O ! s0 p -8# T3 V ) 、 23 . kTV , h ) -( P3 d),Spd 9p、 ; h ), - ( P6 d)Spd ,7 9p, ( P12、15 d)Spd 9p,7 . k W,Spd !. Spd V ?YV(8 =9p、 、 ! 4 h F.N, k # FhM1c M t VCiB, # FhM1c C HW is, V ?Spd F|. HW s , s0 B.B ,T= P I |Q 24-25 .V , (PAO)s H 3H2O2 V ?%Cvs0 Fh1, Q(HR)“d F(SAR)|.V 26.Shen 27?C /,Spd) VE 8NADPH 4,V7 H2O2c 6,4( 0 . 6, (SA)= 6 YVSpm 28. sC.gloeosporioiddes ,) VAh“ s 30,i ?.r1 Ca2+/CaMG |.V 29.Kasukabe 30?C, Spd cDNA E 2= ,Spd Spdc v A9F, H9 、l、 1i j F.cDNA sV , I /,yBty$v c, Y cy0( DREB) ( rd29A).V Spd VT |. o|y0,y F. ID 1 LiB-J( ), FanH-Y(SZ), SunY-Q( l), etal.Accumulationofsalicylicacidincucum-bertreatedwithglucohexaoseanditsrelationtosystemicacquiredresistanceagainstcucumberdownymildew.Ac-taHorticulturaeSinica(), 2005, 32(1):115-117(inChinese) 2 XieB-Y(d), LiH-W( ), FengL-X(P).InductionofresistancetoPhytophthoracapsiceinpepperplantsbyDL-amino-n-bytyricacid.ActaHorticulturaeSinica(), 2002, 29(2):137-140(inChinese) 3 MartinTJ.Metablismandfunctionofpolyaminesinplants:Recentdevelopment(newapproaches).PlantGrowthRegulation, 2001, 34(1):135-148 4 WaldenR, CordeiroA, TiburcioAF.Polyamines:Smallmoleculestriggeringpathwaysinplantgrowthanddevel-opment.PlantPhysiology, 1997, 113:1009-1013 5 Kaur-SawhneyR, TibnrcioAF, GalstonAW.Spermidieandflowerbuddifferentiationinthinlayertobaccotissueculture.Planta, 1993, 173:282-284 6 MurkowskiA.Heatstressandspermidine:Effectonchlorophyllfluorescenceintomatoplants.BiologiaPlantarum, 2001, 44(1):53-577 7 MartinezMA, RamosMG, GardeaAA, etal.Effectofinfiltratedpolyaminesonpolygalacturonaseactivityandchilinginjuryresponsesinzucchinisquash(CucurbitapepoL.).BiochemicalandBiophysicalResearchCom-munications, 2002, 295(1):98-101 8 PapiyaR, KamalaN, SenGuptaDN, etal.Spermidinetreatmenttoriceseedlingsrecoverssalinitystress-in-duceddamageofplasmamembraneandPM-boundH+-ATPaseinsalt-tolerantandsalt-sensitivericecultivars.PlantScience, 2005, 168(3):583-591 9 LiG-M( ), GuoX-L(R ), ShangZ-Q( b), etal.AstudyofrelationshipbetweenPAsandtheresistanceoftomatotoTMV.ActaAgriculturaeBoreali-Sinica( j), 1998, 13(2):64-68(inChinese) 10 CohenSS, BalintR, SindhuRK.Thesynthesisofpoly-aminesfrommethionineinintactanddisruptedleafpro-toplastsofvirus-infectedChinesecabbage.PlantPhysi-ology, 1981, 68:1150-1155 11 PuL( ), KangZ-H( BZ), JiangJ-Z(E ), etal.SiO2 asfungicideagainstBotrytiscinereaoncucumber.ActaPhytopathologicaSinica(h ), 2005, 35(6):186-187(inChinese) 12 JarvisWR.Epidemiology/Coley-SmithJR, VerhoeffK, JarvisWR, eds.TheBiologyofBotrytis.London:AcademicPress, 1980 13 ShangQ-M(i ), ChenS-F( Y), ZhangZ-G(f).Regulationofseleniumonantioxidativeenzymesactivityinpepperleavesunderhightemperaturestress.ActaHorticulturaeSinica(), 2005,8294 i :( h FT 32(1):35-38(inChinese) 14 MoerschbacherBM, NolUM, FlotBE.Ligninbiosyn-theticenzymesinstemrustinfected, resistantandsus-ceptiblenear-isogenicwheatlines.PhysiologicalandMolecularPlantPathology, 1988, 33(1):33-46 15 MillerAR, KeleyTJ, MujerCV.Anodicperoxidaeisoenzymesandpolyphenoloxidaseactivityfromcucum-berfruit:Tissueandsubstratespecificity.Phytochemis-try, 1990, 29(3):705-709 16 ZhuH-B(o), TengB(- b), GaoF-L( ), etal.ChangesofsomebiochemicalcharactersofsoybeanwithdiferentresistantlevelsinfectedbySMV1.ActaAgriculturaeBoreali-OccidentalisSinica( j), 2001, 10(3):38-40(inChinese) 17 MorisonIM.Asemi-micromethodfromthedetermina-tionofligninanditsuseinpredictingthedigestibilityofforagecrops.JournalofScienceFoodAgriculture, 1972,23:455-463 18 HanJ-F(b), ZhuD-H(v), LinX-W( ), etal.Functionofdiferenttreatedmethodswithpolyaminestothegrowthoftobaccoseedlings.To-baccoScience Technology( S/), 1992(1):31-34(inChinese) 19 OuyangG-C( x;), XueY-L( C).Physi-ologicalroleandregulationofphenylpropanoidmetabo-lisminplant.PlantPhysiologyCommunications(3 Y), 1988(3):9-16(inChinese) 20 DeanRA, KucJ.Rapidlignificationinresponsetowoundingandinfectionasamechanismforinducedsys-temicprotectionincucumber.PhysiologicalandMolecu-larPlantPathology, 1987, 31:69-81 21 SongF-M( ), ZhengZ( ), GeX-C(=).ChangesofpolyphenoloxidaseactivityincotonseedlingsafterinfectionbyFusariumoxysporumf.sp.PlantPhysiologyCommunications( 3 Y),1997, 33(3):175-177(inChinese) 22 LuanX-Y( k ), ChenY( ), DuW-G(), etal.Analysisofthechangesofpolyphe-nolicsubstanceinsoybeaninfectedbySMV3.SoybeanScience(v S), 2003, 22(3):186-189(inChi-nese) 23 GuoH-L(R ), ChengG-W(), ChenJ( ), etal.Thefunctionofphenolicmetabolisminresistantmechanismofgrayleafspotofcorn.ActaPhytopathologicaSinica(h ), 2003, 33(4):342-346(inChinese) 24 TassoniA, vanBurenM, FranceschettiM, etal.Poly-aminecontentandmetabolisminArabidopsisthalianaandefectofspermidineonplantdevelopment.PlantPhysiologyandBiochemistry, 2000, 38(5):383-393 25 DuanH-G( S), HuangZ-X(T), LinH-H( ).Theroleofpolyaminesintheontogenyofhigherplants.ActaAgriculturaeBoreali-OccidentalisSinica( j), 2006, 15(2):190-194(inChinese) 26 WaltersD.Resistancetopathogens:Possiblerolesforfreepolyaminesandpolyaminecatabolism.NewPhytolo-gist, 2003, 159(1):109-115 27 ShenWY, NadaK, TachibanaS.Involvementofpoly-aminesinthechillingtoleranceofcucumbercultivars.PlantPhysiology, 2000, 124(1):431-439 28 TakahashiY, BerberichT, MiyazakiA, etal.Sperminesignalingintobacco:Activationofmitogen-activatedproteinkinasesbyspermineismediatedthroughmito-chondrialdysfunction.PlantJournal, 2003, 36:820-829 29 AhnIP, KimS, ChoiWB, etal.Calciumrestorespre-penetrationmorphogenesisabolishedbypolyaminesincolletotrichumgloeosporioidesinfectingredpepper.FEMSMicrobiologyLeters, 2003, 227(2):237-241 30 KasukabeY, HeL, NadaK, etal.Overexpressionofspermidinesynthaseenhancestolerancetomultipleenvi-ronmentalstressesandup-regulatestheexpressionofva-riousstress-regulatedgenesintransgenicArabidopsisthaliana.PlantandCellPhysiology, 2004, 45(6):712-722Te i , 3, 1965 M 3,p V,.1V Yv ? e,?V 60 .E-mail:shangqmmail.caas.net.cn3 I f 830 3 19
展开阅读全文
