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$ iNaHSO3) i(;TYmu1, 2, W2, 3, 4(1.安徽农业大学园艺学院, 安徽合肥 230036;2.上海市农业科学院, 上海市设施园艺技术重点实验室,上海 201106;3.上海交通大学农业与生物学院, 上海 201101;4.上海市宝山区生物技术中心, 上海 201907)K 1:) i ; (;TY# , i Hq/,( Deltastar k, iNaHSO3 A ) 3 (= 。TV : iNaHSO3 V4i(= ; q。 i(NaHSO3) aR i2.0 mmol/LP,( 7( iNaHSO3 A ( V4(= ; q,i ?T3 dP。v( )M1,2.0 mmol/LNaHSO3) 4( ?= ; q20 %P, OX;y0PMST , 9F ATP,7idY= = s PS;r q。c ! =;vf /NaHSO3 ) 。1oM:(; i;T;NaHSO3ms |:Q945.11DS M :AcI|:1007-7146(2009)03-0295-05EffectsofLowConcentrationNaHSO3 TreatmentonPhotosynthesisofCucumberinGreenhousePEIXiao-bo1, 2, LIShi-cheng2, CAIRun3, WANGYue4(1.SchoolofHorticulture, AnhuiAgriculturalUniversity, Hefei230036, Anhui, China;2.ShanghaiAcademyofAgriculturalScience, ShanghaiKeyLaboratoryofProtectedHorticulturalTechnology, Shanghai201106, China;3.CollegeofAgronomyandBiology, ShanghaiJiaotongUniversity, Shanghai201101, China;4.CenterofBiotechnologyofBao-shan, Shanghai201907, China)Abstract:Toprobetheinfluenceandmechanismoflowconcentrationofsulfiteonphotosynthesisofcucumber, onecu-cumbervariety“Deltastar” waschosenandsprayedwithNaHSO3 atleavesinlowconcentrationatdiferentstages, theresultsindicatedthatthephotosynthesisrateofleaveswasimprovedingreenhouse.Theoptimalconcentrationofcucum-berwasabout2.0 mmol/L.Thetreatmentwaseffectivewhencucumberatplantletoratblooming fruitingstage, withcontinuouslyeffectmaintainedforabout3 days.Comparedtothecontrol(sprayingwater), thephotosynthesisrateofcucumberfunctionalleaveswasimprovedby20 %with2.0 mmol/LNaHSO3 treatment, inaccordwithPhenazinemet-hosulfate(PMS)whichisacofactorofcatalyzingcyclic-photophosphroylation, raisingtheATPcontentintheleaves.AndthepotentialphotochemicalefficiencyofPS wasnotafectedbysprayinglowconcentrationofNaHSO3.Applica-tionofNaHSO3 intoprotectedcultureunderinsuficientlightconditionwasalsodiscussedinthearticle.18 3 2009 M6 ; 3 ACTA LASER BIOLOGY SINICA Vol.18 No.3Jun.2009 l :2008-09-15; :2009-01-10 “:SE S/9 “(2008BADCOB02);SE1 S “(39830230); 81 S “(03041106); 8 ! “Te:(1970), 3, a , q,p V,1Z_ ! 。(0Q)peixiaoboahau.edu.cn;peixbsohu.comKeywords:cucumber;greenhouse;photosynthesis;Sodiumbi-sulfite(NaHSO3)T NaHSO34 ;T9F ESX k 1-3 ,BN、r7 j< 3 Vw<zn 。* 4;TNaH-SO3 ; #,i| TB ; 4 P。/ $V 4-9 , iNaHSO3;TrYV PSI0.# ;,9FATP,i ; , d 7,T; 2 3?# A ;g7 T。;T K1 3 V,9 $。T NaHSO3) i# rTX 10-12 , 5 mol/LNaHSO3 ?h #;TY, 100 mg/LNaHSO3r4$ =; c , m 3 200 mg/LNaH-SO3 ?4; q= s c , P=94 。2 mmol/LNaHSO3 V Pl . 9F, P b ? h ,K. c/。YVNaHSO3) i(;TY# ),+Y ! =;v f /NaHSO3) ? i(; q, ? 3 4G 。L, !A9 1“d e, LV( 3B。 i = , ? LC k ; eB,7 O VC L= 3;v f。1 ZEL (CucumissativusL.), i z(Deltastar, RUKZWAAN )。 L2005 M Z j< Sj i。L ,?5 。 uF !9, l u30, 3Q。NaHSO3) sY( 、 7( = NaHSO3。 d?4 、=B = ) , 0 v(CK)。;T; <Y wLLi-6400;N。NaHSO3) (= ; qBWLi-6400;N。ATPc vD 13ZE, | ( ?=,5| “, =sYTv( 0 ) ( 2 mmol/LNaHSO3),Q g | M、 M= ,m ) ( i4 |)10 min, , |B8 A; E ATPc 。= s ; :PAM-2100Lz T;N(Walz, Efeltrich, Germany)。 !; 0/h=C2030-BC= ,M= = s ; 。 /:V s20 min a= ,B l0.1 mol/m2s ;( q600 Hz)v , S;F0;u 7B ;(8 000 mol/m2s, 20 KHz, 0.8 s), a/Kv;Fm;PSIKv;r qFv/Fm/ T9 :Fv/Fm=(Fm-F0)/Fm。 6,3Q。2 Ts2.1 NaHSO3) i(= ; qY iNaHSO3 i(= ,Q 1 = ; q(m1), Tl TM ,) = ; q(Pn) Av, i( NaHSO3 aR i2 mmol/LP。1 2 mmol/LNaHSO3) v= ;T;Y wL(m2), VnNaHSO3), = ;/ ;/; q (。vBHq/sY i( 7( (= 2 mmol/LNaHSO3) ,; <Hq/(m3) ( Vr4; q20 % ,NT V3 dP(m4)。2.2 NaHSO3PMS) (= ; qY= y;4ATP ? 17;TKy0,|b;y0 ; Jq(PMS)= V9F= ATPc 4;r q。296 ; 3 18 ( 7 ,2.0 mmol/LNaHSO3) (= ,2?10:00ATPc (mol/m2)5.12 0.37,7v3.01 0.23(P=0.033,sA)。1 2.0 mmol/LNaHSO30.2mol/LPMSsY) ) (= ; qY(m5),TV , sY) ) ( V; q20 %P,sY) ) 4; qM ,V T ( V ? 9F ATP,y7 ) VCFr。|b;y0 ; Jq(PMS) = V9F= ATPc 4;r q。2973 mu: iNaHSO3) i(;TY NaHSO3处理浓度为 2.0 mmol/L, PMS浓度为 0.2mol/L,测定光强为 1 000 mol/m2.s。Treatedwith2 mmol/LNaHSO3 and/orconcentrationofPMS0.2 mol/L, measuredwithlightintensityof1 000 molphotons/ms.图 5 花期NaHSO3和PMS处理对黄瓜叶片净光合速率的影响Fig.5 TheeffectofNaHSO3 andPMSonnetphotosynthsisrateincucumberleafatbloomstage2.3 NaHSO3) (= = s ;Y= s ; Fv/FmT PS1; , QPS;r q。 m6 U, 0.5 mmol/L 4.0 mmol/LNaHSO3) i(= s Fv/FmYA,V PSidiNaHSO3) Y。CK, A, B, C, D, E分别表示对照, 0.5 mmol/L, 1.0mmol/L, 2.0 mmol/L, 3.0 mmol/L和 4.0 mmol/LNaHSO3处理。CK, A, B, C, DandErepresentcontrol, andtreatedwithNaHSO3 atconcentration(mmol/L)of0.5, 1.0, 2.0.3.0and4.0 respectively.图 6 黄瓜花期 NaHSO3处理对 PS光化学效率(Fv/Fm)的影响Fig.6 TheefectofNaHSO3 onthepotentialphotochemi-caleficiency(Fv/Fm)ofPS incucumberatbloomstage3 ) T iNaHSO3; qT, 8 L8 = L ( L 4-6 ,NaH-SO34; q “ = =;,9F = s8ATP ? ,1By PS T0. 9 。 - 14-15 ,= y;4ATP ? 17;TKy0,|b;y0 ; Jq(PMS) = V9F= ATPc 4;r q。 LNaHSO3X V;y0PMS ) (= ; q4Fr, L NaHSO3) = 4; qy1 ;9FATP#。 2Z ! 37,+Y C i,=1 i =KB20 P,C;v |? ,;v ( v/, K1Ky0 16 。V' LT A, i 3 ( 7( ,= 2 mmol/LNaHSO3(rT, V4 ; q20 %P; i =;v(PAR) , iNaHSO3) (rT, OPAR200 mol/m2s H,NaHSO3) 4= ; qrTF A(m3、m4)。yN, ! 3 iNaHSO3 ?4 = ; q,7 Oh ! =;v 3 Y4 BHo。References 1 P,B Y. ; _ ) N0 3?Y J.j</ , 1993, 4:30-32.XUYu-xiang, TIANShu-fang.EfectofNaHSO3 onGrowthandDevelopmentofEggplant J.AgronomyandTechnology,1993, 4:30-32. 2ld,k. ; _ )$ J.T S, 1991, 8(2):97-99.WANGYi-zhang, WUXiao-dong.AppliedResearchofSodiumBisulfiteonDangshanPear J.FruitScience, 1991, 8(2):97-99. 3p. , O. ; _ ) m 3 J.S jY, 2000, 16(4):15-17.TUChuan-lin, KETan.ImprovementofTeaYieldandQualitybyNaHSO3 inHilAreaJ.ChineseAgriculturalScienceBulle-tin, 2000, 16(4):15-17. 4L, + . ; _ );# YJ. 3 , 1987, 13:42-50.298 ; 3 18 TANShi, SHENYun-gang.TheEfectofSodiumBisulfiteonPhotosyntheticApparatusandItsOperationJ.Phytophysiologi-ca, 1987, 13:42-50. 5E , + , 2,. ; _ ); </= s8;TJ. 3 , 1989,15:101-104.WEIJia-mian, SHENYun-gang, LIDe-yao, etal.StimulatoryEfectofCyclic-photophosphorylationofChloroplastsunderLowLightIntensity J.ActaPhytophysiologicaSinica, 1989, 15:101-104. 6,E . i ; _ ) Vl = ;TJ. 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Z: Z S/, 1985:115-117.WANGWei-guang.PhytophysiologicalExperimentsHandbookM.Shanghai:ShanghaiSciencePublishingHouse, 1985:115-117. 14, 2, + . i ; Jq(PMS)=;bTJ. 3 , 1989, 15(3):307-312.XUBao-ji, LIDe-yao, SHENYun-gang.TheEnhancementofLowConcentrationofPMSonLeaf' sPhotosyntheticOxgenEvo-lutioninLeafJ.ActaPhytophsiologicalSinica, 1989, 15(3):307-312. 15R ,f, + .|) V=c = ZE)J. 3 Y, 1994, 30(5):360-362.GUOLian-wang, ZHANGYan, SHENYun-gang.StudyontheMethodofFeedingSubstancetoLeavesThroughPetioles J.PlantPhysiologyCommunication, 1994, 30(5):360-362. 16 mu, W, ,.C i;+J. j<v, 2005, 32(2):246-249.PEIXiao-bo, LIShi-cheng, CAIRun, etal.StudiesontheCharacteristicofLightEnvironmentinModernGreenhouseJ.JournalofAnhuiAgriculturalUniversity, 2005, 32(2):246-249.2993 mu: iNaHSO3) i(;TY
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