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Abstract 1Identification and characterization the function andsynthetic pathway of maize natural product isoorientin Article Title Font Times New Roman Size 3 LineSpacing Double Spacing Xiaorong Sun Zhongbao Zhang Author Author 1 Author 2 Author 3 Font Times New Roman Font size 12pt Linespacing Double line spacing Institute of Biotechnology BeijingAcademy ofAgriculture and Forestry Sciences Beijing China 100097 Author s Affiliation Font Times New Roman Font Size Small 5 Line Spacing DoubleSpacing Abstract Objective Plant flavonoids are beneficial to human health Isoorientin a naturalC glycoside flavone plays an important role in resistance of human diseases such asanti inflammatory antioxidative effects and anti cancer cell proliferation Although differentclasses of flavonoid have been well characterized in plants the genes involved in the biosynthesisof isoorientin are largely unknown in maize Method Here we used targeted metabolic profilingof isoorientin on the silks in an association panel consisting of 294 maize inbred lines andidentified a gene ZmCGT1 by genome wide association analysis GWAS Result The ZmCGT1protein was characterized as a 2 hydroxyflavanone C glycosyltransferase and can C glycosylate 2 hydroxyflavanone to form flavone C glycoside after dehydration Moreover overexpressingZmCGT1 increased isoorientin level and RNA mediated interference ZmCGT1 decreasedaccumulation of isoorientin in maize silk Further two nucleotide polymorphisms A502C andA1022G which led to amino acid changes I168L and E341G respectively were identified to befunctional polymorphisms responsible for natural variation of isoorientin Conclusion Takentogether we identified a gene ZmCGT1 which plays an important role in isoorientin biosynthesisand provides the insights of the genetic basis of the natural variations of isoorientin level in maizesilk The identified favorable alleles CG of ZmCGT1 may be further used for geneticimprovement of nutritional quality in maize Body text Font Times New Roman Font size 5 Line spacing Double spacin Key words Maize silk Isoorientin C glycosyltransferase Functional polymorphisms Key words Font Times New Roman Font size 5 Line spacing single line spacing
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